With the accumulation of a lot of protein sequences, it has become extremely important to look for or design sequences which will lead to enhanced protein expression. A very common method is to incorporate multiple copies of genes in the host organism. The problem in this approach is that multiple copies of the same gene can lead to homologus recombination and ultimately might result in silencing of the gene.
Terai G. et. al.(2017) in their recent article have designed protein coding sequences that perform this same function without causing allteration of function. The method is based on a genetic algorithm. Their method takes care of the gene copy number as well as the codon usage frequencies. The method is known as the Tandem designer and is available at the following URL: http://tandem.trahed.jp/tandem/
Reference:
Terai, G. et. al.(2017) Evolutionary Design of the multiple genes encoding the same protein. Bioinformatics 33(11):1613-1620.
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