Relative fitness of mutants on a large scale has been done by sequencing the transposon insertion libraries.Transposons have been used earlier also for gene expression studies. However there is a lack of software tools for analyzing the data generated in this area.
Abramova A. et.al.(2021) have created a new software tool called CAFE or Coefficient-based Analysis of Fitness by read Enrichment which analyzes such data. It creates a fitness coefficient for computation of each gene condition.The fitness coefficients are later analyzed statistically.
The software has been made in R and Perl and can be accessed at the following URL: https://github.com/bengtssonpalme/cafe and the other URL: http://microbiology.se/software/cafe/
Reference:
Abramova A. et. al. (2021) CAFE: a software suite for analysis of paired-sample transposon insertion sequencing data. Bioinformatics 37(1):121-122.